Identification of the key gene and pathways associated with osteoarthritis via single-cell RNA sequencing on synovial fibroblasts.

ABSTRACT: Osteoarthritis (OA) is a chronic degenerative joint disease with its onset closely related to the growth of synovial fibroblasts (SFs), yet the genes involved in are few reported. In our study, we aimed to identify the OA-associated key gene and pathways via the single-cell RNA sequencing (scRNA-seq) analysis on SFs.scRNA-seq data of SFs from OA sufferers were accessed from GEO database, then the genes involved in were subjected to principal component analysis (PCA) and T-Stochastic Neighbor Embedding (TSNE) Analysis. GO and KEGG enrichment analyses were performed to find the most enriched functions and pathways associated with marker genes and a PPI network was constructed to identify the key gene associated with OA occurrence.Findings revealed that marker genes in three cell types identi

SUBMITTER: Wu Z 

PROVIDER: S-EPMC7437759 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature