Conditionally essential genes for survival during starvation in Enterococcus faecium E745.

ABSTRACT:

Background

The nosocomial pathogen Enterococcus faecium can survive for prolonged periods of time on surfaces in the absence of nutrients. This trait is thought to contribute to the ability of E. faecium to spread among patients in hospitals. There is currently a lack of data on the mechanisms that are responsible for the ability of E. faecium to survive in the absence of nutrients.

Results

We performed a high-throughput transposon mutant library screening (Tn-seq) to identify genes that have a role in long-term survival during incubation in phosphate-buffered saline (PBS) at 20 °C. A total of 24 genes were identified by Tn-seq to contribute to survival in PBS, with functions associated with the general stress response, DNA repair, metabolism, and membrane homeostasis. The g

SUBMITTER: de Maat V 

PROVIDER: S-EPMC7437932 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature