Reducing WBC background in cancer cell separation products by negative acoustic contrast particle immuno-acoustophoresis.

ABSTRACT: Cancer cells display acoustic properties enabling acoustophoretic separation from white blood cells (WBCs) with 2-3 log suppression of the WBC background. However, a subset of WBCs has overlapping acoustic properties with cancer cells, which is why label-free acoustophoretic cancer cell isolation needs additional purification prior to analysis. This paper reports for the first time a proof of concept for continuous flow acoustophoretic negative selection of WBCs from cancer cells using negative acoustic contrast elastomeric particles (EPs) activated with CD45-antibodies that specifically bind to WBCs. The EP/WBC complexes align at the acoustic pressure anti-nodes along the channel walls while unbound cancer cells focus to the pressure node in the channel center, enabling continuous flow ba

SUBMITTER: Cushing K 

PROVIDER: S-EPMC7437979 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature