Mass Defect-Based DiLeu Tagging for Multiplexed Data-Independent Acquisition.

ABSTRACT: The unbiased selection of peptide precursors makes data-independent acquisition (DIA) an advantageous alternative to data-dependent acquisition (DDA) for discovery proteomics, but traditional multiplexed quantification approaches employing mass difference labeling or isobaric tagging are incompatible with DIA. Here, we describe a strategy that permits multiplexed quantification by DIA using mass defect-based N,N-dimethyl leucine (mdDiLeu) tags and high-resolution tandem mass spectrometry (MS²) analysis. Millidalton mass differences between mdDiLeu isotopologues produce fragment ion multiplet peaks separated in mass by as little as 5.8 mDa, enabling up to 4-plex quantification in DIA MS² spectra. Quantitative analysis of yeast samples displayed comparable

SUBMITTER: Zhong X 

PROVIDER: S-EPMC7438256 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature