WTAP promotes osteosarcoma tumorigenesis by repressing HMBOX1 expression in an m⁶A-dependent manner.

ABSTRACT: N⁶-methyladenosine (m⁶A) regulators are involved in the progression of various cancers via regulating m⁶A modification. However, the potential role and mechanism of the m⁶A modification in osteosarcoma remains obscure. In this study, WTAP was found to be highly expressed in osteosarcoma tissue and it was an independent prognostic factor for overall survival in osteosarcoma. Functionally, WTAP, as an oncogene, was involved in the proliferation and metastasis of osteosarcoma in vitro and vivo. Mechanistically, M⁶A dot blot, RNA-seq and MeRIP-seq, MeRIP-qRT-PCR and luciferase reporter assays showed that HMBOX1 was identified as the target gene of WTAP, which regulated HMBOX1 stability depending on m⁶A modification at the 3'UTR

SUBMITTER: Chen S 

PROVIDER: S-EPMC7438489 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature