Lung SOD3 limits neurovascular reperfusion injury and systemic immune activation following transient global cerebral ischemia.

ABSTRACT:

Background and objectives

Studies implicate the lung in moderating systemic immune activation via effects on circulating leukocytes. In this study, we investigated whether targeted expression of the antioxidant extracellular superoxide dismutase (SOD3) within the lung would influence post-ischemic peripheral neutrophil activation and CNS reperfusion injury.

Methods

Adult, male mice expressing human SOD3 within type II pneumocytes were subjected to 15 min of transient global cerebral ischemia. Three days post-reperfusion, lung and brain tissue was collected and analyzed by immunohistochemistry for inflammation and injury markers. In vitro motility and neurotoxicity assays were conducted to ascertain the direct effects of hSOD3 on PMN activation. Results were compared against

SUBMITTER: Mai N 

PROVIDER: S-EPMC7438610 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature