Endoplasmic Reticulum Lumenal Indicators in <i>Drosophila</i> Reveal Effects of HSP-Related Mutations on Endoplasmic Reticulum Calcium Dynamics.

ABSTRACT: Genes for endoplasmic reticulum (ER)-shaping proteins are among the most commonly mutated in hereditary spastic paraplegia (HSP). Mutation of these genes in model organisms can lead to disruption of the ER network. To investigate how the physiological roles of the ER might be affected by such disruption, we developed tools to interrogate its Ca²⁺ signaling function. We generated GAL4-driven Ca²⁺ sensors targeted to the ER lumen, to record ER Ca²⁺ fluxes in identified Drosophila neurons. Using GAL4 lines specific for Type Ib or Type Is larval motor neurons, we compared the responses of different lumenal indicators to electrical stimulation, in axons and presynaptic terminals. The most effective sensor, ER-GCaMP6-210, had a Ca²⁺ affin

SUBMITTER: Oliva MK 

PROVIDER: S-EPMC7438849 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature