Comprehensive Proteomic Analysis of Lysine Ubiquitination in Seedling Leaves of Nicotiana tabacum.

ABSTRACT: Lysine ubiquitination, a widely studied posttranslational modification, plays vital roles in various biological processes in eukaryotic cells. Although several studies have examined the plant ubiquitylome, no such research has been performed in tobacco, a model plant for molecular biology. Here, we comprehensively analyzed lysine ubiquitination in tobacco (Nicotiana tabacum) using LC-MS/MS along with highly sensitive immune-affinity purification. In total, 964 lysine-ubiquitinated (K^ub) sites were identified in 572 proteins. Extensive bioinformatics studies revealed the distribution of these proteins in various cellular locations, including the cytoplasm, chloroplast, nucleus, and plasma membrane. Notably, 25% of the K^ub proteins were located in the chloroplast

SUBMITTER: Zhan H 

PROVIDER: S-EPMC7439365 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature