A molecular-beacon-based asymmetric PCR assay for detecting polymorphisms related to folate metabolism.

ABSTRACT:

Background

Polymorphisms (rs1801133 or C677T; rs1801131 or A1298C) of the MTHFR gene and rs1801394 (A66G) of the MTRR gene are important genetic determinants of folate metabolism. A convenient, sensitive, and reliable method is required to detect polymorphisms for the precise supplementation of folate.

Methods

A rapid detection method based on molecular beacon probes that can detect rs1801133, rs1801131, and rs1801394 simultaneously was developed in this study. Specific primers and probes were designed, and the amplification system and conditions were optimized. We applied our method to a group of 500 unrelated women of gestational age in the Dongguan region of Guangdong Province in China. The clinical performance of this assay was evaluated by testing 94 samples in comparis

SUBMITTER: Peng Q 

PROVIDER: S-EPMC7439423 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature