Structure and Multitasking of the c-di-GMP-Sensing Cellulose Secretion Regulator BcsE.

ABSTRACT: Most bacteria respond to surfaces by biogenesis of intracellular c-di-GMP, which inhibits motility and induces secretion of biofilm-promoting adherence factors. Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties. We recently showed that in Escherichia coli, most Bcs proteins form a megadalton-sized secretory nanomachine, but the role and structure of individual regulatory components remained enigmatic. Here, we demonstrate that essential-for-secretion BcsR and BcsQ regulate each oth

SUBMITTER: Zouhir S 

PROVIDER: S-EPMC7439463 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature