Phosphorylation-Dependent Assembly of a 14-3-3 Mediated Signaling Complex during Red Blood Cell Invasion by Plasmodium falciparum Merozoites.

ABSTRACT: Red blood cell (RBC) invasion by Plasmodium merozoites requires multiple steps that are regulated by signaling pathways. Exposure of P. falciparum merozoites to the physiological signal of low K⁺, as found in blood plasma, leads to a rise in cytosolic Ca²⁺, which mediates microneme secretion, motility, and invasion. We have used global phosphoproteomic analysis of merozoites to identify signaling pathways that are activated during invasion. Using quantitative phosphoproteomics, we found 394 protein phosphorylation site changes in merozoites subjected to different ionic environments (high K⁺/low K⁺), 143 of which were Ca²⁺ dependent. These included a number of signaling proteins such as catalytic and regulatory subunits of

SUBMITTER: More KR 

PROVIDER: S-EPMC7439480 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature