Isolation of novel citrus and plum fruit promoters and their functional characterization for fruit biotechnology.

ABSTRACT:

Background

Promoters that confer expression in fruit tissues are important tools for genetic engineering of fruit quality traits, yet few fruit-specific promoters have been identified, particularly for citrus fruit development.

Results

In this study, we report five citrus fruit-specific/preferential promoters for genetic engineering. Additionally, we have characterized a novel fruit-preferential promoter from plum. Genes specifically expressed in fruit tissues were selected and their isolated promoter regions were fused with the GUSPlus reporter gene for evaluation in transgenic plants. Stable transformation in Micro-Tom tomato demonstrated that the candidate promoter regions exhibit differing levels of expression and with varying degrees of fruit specificity.

Conclusions

SUBMITTER: Dasgupta K 

PROVIDER: S-EPMC7439555 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature