Cis and trans effects differentially contribute to the evolution of promoters and enhancers.

ABSTRACT:

Background

Gene expression differences between species are driven by both cis and trans effects. Whereas cis effects are caused by genetic variants located on the same DNA molecule as the target gene, trans effects are due to genetic variants that affect diffusible elements. Previous studies have mostly assessed the impact of cis and trans effects at the gene level. However, how cis and trans effects differentially impact regulatory elements such as enhancers and promoters remains poorly understood. Here, we use massively parallel reporter assays to directly measure the transcriptional outputs of thousands of individual regulatory elements in embryonic stem cells and measure cis and trans effects between human and mouse.

Results

Our approach reveals that cis effects are wide

SUBMITTER: Mattioli K 

PROVIDER: S-EPMC7439725 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature