Detecting DNA and RNA and Differentiating Single-Nucleotide Variations via Field-Effect Transistors.

ABSTRACT: We detect short oligonucleotides and distinguish between sequences that differ by a single base, using label-free, electronic field-effect transistors (FETs). Our sensing platform utilizes ultrathin-film indium oxide FETs chemically functionalized with single-stranded DNA (ssDNA). The ssDNA-functionalized semiconducting channels in FETs detect fully complementary DNA sequences and differentiate these sequences from those having different types and locations of single base-pair mismatches. Changes in charge associated with surface-bound ssDNA vs double-stranded DNA (dsDNA) alter FET channel conductance to enable detection due to differences in DNA duplex stability. We illustrate the capability of ssDNA-FETs to detect complementary RNA sequences and to distinguish from RNA sequences with sin

SUBMITTER: Cheung KM 

PROVIDER: S-EPMC7439785 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature