A Human IgSF Cell-Surface Interactome Reveals a Complex Network of Protein-Protein Interactions.

ABSTRACT: Cell-surface protein-protein interactions (PPIs) mediate cell-cell communication, recognition, and responses. We executed an interactome screen of 564 human cell-surface and secreted proteins, most of which are immunoglobulin superfamily (IgSF) proteins, using a high-throughput, automated ELISA-based screening platform employing a pooled-protein strategy to test all 318,096 PPI combinations. Screen results, augmented by phylogenetic homology analysis, revealed ∼380 previously unreported PPIs. We validated a subset using surface plasmon resonance and cell binding assays. Observed PPIs reveal a large and complex network of interactions both within and across biological systems. We identified new PPIs for receptors with well-characterized ligands and binding partners for "orphan" receptors. N

SUBMITTER: Wojtowicz WM 

PROVIDER: S-EPMC7440162 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature