Extracellular glutamate and IDH1^R132H inhibitor promote glioma growth by boosting redox potential.

ABSTRACT:

Purpose

Somatic mutations of the isocitrate dehydrogenase 1 (IDH1) gene, mostly substituting Arg132 with histidine, are associated with better patient survival, but glioma recurrence and progression are nearly inevitable, resulting in disproportionate morbidity and mortality. Our previous studies demonstrated that in contrast to hemizygous IDH1^R132H (loss of wild-type allele), heterozygous IDH1^R132H is intrinsically glioma suppressive but its suppression of three-dimensional (3D) growth is negated by extracellular glutamate and reducing equivalent. This study sought to understand the importance of 3D culture in IDH1^R132H biology and the underlying mechanism of the glutamate effect.

Methods

RNA sequencing data of IDH1^R132H-heterozyg

SUBMITTER: Tiburcio PDB 

PROVIDER: S-EPMC7440756 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature