Coronavirus discovery by metagenomic sequencing: a tool for pandemic preparedness.

ABSTRACT:

Introduction

The SARS-CoV-2 pandemic of 2020 is a prime example of the omnipresent threat of emerging viruses that can infect humans. A protocol for the identification of novel coronaviruses by viral metagenomic sequencing in diagnostic laboratories may contribute to pandemic preparedness.

Aim

The aim of this study is to validate a metagenomic virus discovery protocol as a tool for coronavirus pandemic preparedness.

Methods

The performance of a viral metagenomic protocol in a clinical setting for the identification of novel coronaviruses was tested using clinical samples containing SARS-CoV-2, SARS-CoV, and MERS-CoV, in combination with databases generated to contain only viruses of before the discovery dates of these coronaviruses, to mimic virus discovery.

Result

SUBMITTER: Carbo EC 

PROVIDER: S-EPMC7441049 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature