Characterization of methicillin-resistant Staphylococcus aureus through genomics approach.

ABSTRACT: In the present study, a total of 35 S. aureus isolates collected from two different geographical locations viz., Germany and Hungary were tested for their methicillin-resistant phenotype which revealed a high incidence of methicillin-resistant S. aureus. The quantitative test for biofilm production revealed that 73.3% of isolates were biofilm producers. The isolates were further characterized using a set of biochemical and genotypic methods such as amplification and analysis of S. aureus species-specific sequence and mecA gene. The 33 mecA positive isolates were then characterized by the amplification of SCCmec and pvl toxin genes. Further, based on the biofilm-forming phenotype, 15 isolates were selected and characterized through PCR-RFLP o

SUBMITTER: Naorem RS 

PROVIDER: S-EPMC7441129 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature