An atlas of O-linked glycosylation on peptide hormones reveals diverse biological roles.

ABSTRACT: Peptide hormones and neuropeptides encompass a large class of bioactive peptides that regulate physiological processes like anxiety, blood glucose, appetite, inflammation and blood pressure. Here, we execute a focused discovery strategy to provide an extensive map of O-glycans on peptide hormones. We find that almost one third of the 279 classified peptide hormones carry O-glycans. Many of the identified O-glycosites are conserved and are predicted to serve roles in proprotein processing, receptor interaction, biodistribution and biostability. We demonstrate that O-glycans positioned within the receptor binding motifs of members of the neuropeptide Y and glucagon families modulate receptor activation properties and substantially extend peptide half-lives. Our study highlights the importanc

SUBMITTER: Madsen TD 

PROVIDER: S-EPMC7441158 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature