Melanoblasts Populate the Mouse Choroid Earlier in Development Than Previously Described.

ABSTRACT:

Purpose

Human choroidal melanocytes become evident in the last trimester of development, but very little is known about them. To better understand normal and diseased choroidal melanocyte biology we examined their precursors, melanoblasts (MB), in mouse eyes during development, particularly their relation to the developing vasculature and immune cells.

Methods

Naïve B6(Cg)-Tyrc-2J/J albino mice were used between embryonic (E) day 15.5 and postnatal (P) day 8, with adult controls. Whole eyes, posterior segments, or dissected choroidal wholemounts were stained with antibodies against tyrosinase-related protein 2, ionized calcium binding adaptor molecule-1 or isolectin B4, and examined by confocal microscopy. Immunoreactive cell numbers in the choroid were quantified with Imari

SUBMITTER: McMenamin PG 

PROVIDER: S-EPMC7441366 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature