Response of the gut microbiota during the Clostridioides difficile infection in tree shrews mimics those in humans.

ABSTRACT:

Background

Clostridioides difficile is a major cause of antibiotic associated diarrhea. Several animal models are used to study C. difficile infection (CDI). The tree shrew has recently been developed as a model of primate processes. C. difficile infection has not been examined in tree shrews. We infected tree shrews with hyper-virulent C. difficile strains and examined the alterations in gut microbiota using 16S rRNA gene sequencing.

Results

C. difficile colonized the gastrointestinal tract of tree shrew and caused diarrhea and weight loss. Histopathologic examination indicated structures and mucosal cell destruction in ileal and colonic tissues. The gut microbial community was highly diversity before infection and was dominated by Firmicutes, Fusobacteria, Bacteroidetes, a

SUBMITTER: Gu W 

PROVIDER: S-EPMC7441558 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature