Development of Caco-2 cells expressing four CYPs via a mammalian artificial chromosome.

ABSTRACT:

Background

Oral administration is the most common way to deliver drugs to the systemic circulation or target organs. Orally administered drugs are absorbed in the intestine and metabolized in the intestine and liver. In the early stages of drug development, it is important to predict first-pass metabolism accurately to select candidate drugs with high bioavailability. The Caco-2 cell line derived from colorectal cancer is widely used as an intestinal model to assess drug membrane permeability. However, because the expression of major drug-metabolizing enzymes, such as cytochrome P450 (CYP), is extremely low in Caco-2 cells, it is difficult to predict intestinal metabolism, which is a significant factor in predicting oral drug bioavailability. Previously, we constructed a mouse arti

SUBMITTER: Ohta Y 

PROVIDER: S-EPMC7441628 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature