Significance of hub genes and immune cell infiltration identified by bioinformatics analysis in pelvic organ prolapse.

ABSTRACT:

Objective

Pelvic organ prolapse (POP) refers to the decline of pelvic organ position and dysfunction caused by weak pelvic floor support. The aim of the present study was to screen the hub genes and immune cell infiltration related to POP disease.

Methods

Microarray data of 34 POP tissues in the GSE12852 gene expression dataset were used as research objects. Weighted gene co-expression network analysis (WGCNA) was performed to elucidate the hub module and hub genes related to POP occurrence. Gene function annotation was performed using the DAVID tool. Differential analysis based on the GSE12852 dataset was carried out to explore the expression of the selected hub genes in POP and non-POP tissues, and RT-qPCR was used to validate the results. The differential immune cell infi

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC7441923 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature