Three-Dimensional Traction Microscopy with a Fiber-Based Constitutive Model.

ABSTRACT: Tractions exerted by cells on their surroundings play an important role in many biological processes including stem cell differentiation, tumorigenesis, cell migration, cancer metastasis, and angiogenesis. The ability to quantify these tractions is important in understanding and manipulating these processes. Three-dimensional traction force microscopy (3DTFM) provides reliable means of evaluating cellular tractions by first measuring the displacement of fluorescent beads in response to these tractions in the surrounding matrix, and then using this measurement to compute the tractions. However, most applications of 3DTFM assume that the surrounding extra-cellular matrix (ECM) is non-fibrous, despite the fact that in many natural and synthetic environments the ECM contains a significant prop

SUBMITTER: Song D 

PROVIDER: S-EPMC7442266 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature