Generation of diffuse intrinsic pontine glioma mouse models by brainstem-targeted in utero electroporation.

ABSTRACT:

Background

Diffuse intrinsic pontine gliomas (DIPGs) are highly lethal childhood brain tumors. Their unique genetic makeup, pathological heterogeneity, and brainstem location all present challenges to treatment. Developing mouse models that accurately reflect each of these distinct features will be critical to advance our understanding of DIPG development, progression, and therapeutic resistance. The aims of this study were to generate new mouse models of DIPG and characterize the role of specific oncogenic combinations in DIPG pathogenesis.

Methods

We used in utero electroporation (IUE) to transfect neural stem cells in the developing brainstem with PiggyBac DNA transposon plasmids. Combinations of platelet-derived growth factor B (PDGFB), PdgfraD842V, or PdgfraWT, combined

SUBMITTER: Patel SK 

PROVIDER: S-EPMC7442382 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature