A functionally neutral single chain antibody to measure beta-1 integrin uptake and recycling.

ABSTRACT: Integrin-mediated cell adhesion and signaling are critical for many physiological processes. The dynamic turnover of integrins and their associated adhesion complexes through endocytic and recycling pathways has emerged as an important mechanism for controlling cell migration and invasion in cancer. Thus, the regulation of integrin trafficking and how this may be altered by disease-specific molecular mechanisms has generated considerable interest. However, current tools available to study integrin trafficking may cause artifacts and/or do not provide adequate kinetic information. Here, we report the generation of a functionally neutral and monovalent single chain antibody to quantitatively and qualitatively measure β1 integrin trafficking in cells. Our novel probe can be used in a variety

SUBMITTER: Lakoduk AM 

PROVIDER: S-EPMC7442622 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature