Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection.

ABSTRACT: The full neutrophil heterogeneity and differentiation landscape remains incompletely characterized. Here, we profiled >25,000 differentiating and mature mouse neutrophils using single-cell RNA sequencing to provide a comprehensive transcriptional landscape of neutrophil maturation, function and fate decision in their steady state and during bacterial infection. Eight neutrophil populations were defined by distinct molecular signatures. The three mature peripheral blood neutrophil subsets arise from distinct maturing bone marrow neutrophil subsets. Driven by both known and uncharacterized transcription factors, neutrophils gradually acquire microbicidal capability as they traverse the transcriptional landscape, representing an evolved mechanism for fine-tuned regulation of an effective but

SUBMITTER: Xie X 

PROVIDER: S-EPMC7442692 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature