Reelin signaling modulates GABA_B receptor function in the neocortex.

ABSTRACT: Reelin is a protein that is best known for its role in controlling neuronal layer formation in the developing cortex. Here, we studied its role for post-natal cortical network function, which is poorly explored. To preclude early cortical migration defects caused by Reelin deficiency, we used a conditional Reelin knock-out (Reln^cKO ) mouse, and induced Reelin deficiency post-natally. Induced Reelin deficiency caused hyperexcitability of the neocortical network in vitro and ex vivo. Blocking Reelin binding to its receptors ApoER2 and VLDLR resulted in a similar effect. Hyperexcitability in Reln^cKO organotypic slice cultures could be rescued by co-culture with wild-type organotypic slice cultures. Moreover, the GABA_B receptor (GABA_B R) agonist bacl

SUBMITTER: Hamad MIK 

PROVIDER: S-EPMC7442713 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature