Biomarkers Associated with Beneficial PD-1 Checkpoint Blockade in Non-Small Cell Lung Cancer (NSCLC) Identified Using High-Plex Digital Spatial Profiling.

ABSTRACT:

Purpose

Only a minority of patients with advanced non-small cell lung cancer (NSCLC) truly benefits from single-agent PD-1 checkpoint blockade, and more robust predictive biomarkers are needed.

Experimental design

We assessed tumor samples from 67 immunotherapy-treated NSCLC cases represented in a tissue microarray, 53 of whom had pretreatment samples and received monotherapy. Using GeoMx Digital Spatial Profiling System (NanoString Technologies), we quantified 39 immune parameters simultaneously in four tissue compartments defined by fluorescence colocalization [tumor (panCK⁺), leucocytes (CD45⁺), macrophages (CD68⁺), and nonimmune stroma].

Results

A total of 156 protein variables were generated per case. In the univariate unadjusted

SUBMITTER: Zugazagoitia J 

PROVIDER: S-EPMC7442721 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature