Astaxanthin inhibits the reduction of glycolysis during the activation of hepatic stellate cells.

ABSTRACT:

Aims

Hepatic stellate cells (HSCs) play an essential role in the development of liver fibrosis by producing extracellular matrix proteins, growth factors, and pro-inflammatory and pro-fibrogenic cytokines once activated. We previously demonstrated that astaxanthin (ASTX), a xanthophyll carotenoid, attenuates HSC activation. The objective of this study was to investigate whether there is a difference in glycolysis between quiescent and activated HSCs and the effect of ASTX on glycolysis during HSC activation.

Materials and methods

Mouse primary HSCs were activated for 7 days in the presence or absence of 25 μM of ASTX. Quiescent HSCs (qHSCs), 1 day after isolation, and activated HSCs (aHSCs) treated with/without ASTX were plated in a Seahorse XF24 cell culture microplate for

SUBMITTER: Bae M 

PROVIDER: S-EPMC7442723 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature