Mapping systemic lupus erythematosus heterogeneity at the single-cell level.

ABSTRACT: Patients with systemic lupus erythematosus (SLE) display a complex blood transcriptome whose cellular origin is poorly resolved. Using single-cell RNA sequencing, we profiled ~276,000 peripheral blood mononuclear cells from 33 children with SLE with different degrees of disease activity and 11 matched controls. Increased expression of interferon-stimulated genes (ISGs) distinguished cells from children with SLE from healthy control cells. The high ISG expression signature (ISG^hi) derived from a small number of transcriptionally defined subpopulations within major cell types, including monocytes, CD4⁺ and CD8⁺ T cells, natural killer cells, conventional and plasmacytoid dendritic cells, B cells and especially plasma cells. Expansion of unique subpopulations

SUBMITTER: Nehar-Belaid D 

PROVIDER: S-EPMC7442743 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature