Taf14 recognizes a common motif in transcriptional machineries and facilitates their clustering by phase separation.

ABSTRACT: Saccharomyces cerevisiae TBP associated factor 14 (Taf14) is a well-studied transcriptional regulator that controls diverse physiological processes and that physically interacts with at least seven nuclear complexes in yeast. Despite multiple previous Taf14 structural studies, the nature of its disparate transcriptional regulatory functions remains opaque. Here, we demonstrate that the extra-terminal (ET) domain of Taf14 (Taf14_ET) recognizes a common motif in multiple transcriptional coactivator proteins from several nuclear complexes, including RSC, SWI/SNF, INO80, NuA3, TFIID, and TFIIF. Moreover, we show that such partner binding promotes liquid-liquid phase separation (LLPS) of Taf14_ET, in a mechanism common to YEATS-associated ET domains (e.g., AF9_ET)

SUBMITTER: Chen G 

PROVIDER: S-EPMC7442819 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature