Investigating Transfusion-related Sepsis Using Culture-Independent Metagenomic Sequencing.

ABSTRACT:

Background

Transfusion-related sepsis remains an important hospital infection control challenge. Investigation of septic transfusion events is often restricted by the limitations of bacterial culture in terms of time requirements and low yield in the setting of prior antibiotic administration.

Methods

In 3 gram-negative septic transfusion cases, we performed metagenomic next-generation sequencing (mNGS) of direct clinical blood specimens in addition to standard culture-based approaches utilized for infection control investigations. Pathogen detection leveraged IDSeq, a new open-access microbial bioinformatics portal. Phylogenetic analysis was performed to assess microbial genetic relatedness and understand transmission events.

Results

mNGS of direct clinical blood spe

SUBMITTER: Crawford E 

PROVIDER: S-EPMC7442849 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature