Superoxide-induced Type I collagen secretion depends on prolyl 4-hydroxylases.

ABSTRACT: Reactive oxygen species (ROS) including superoxide (O₂^•-) play an important role in a variety of diseases, including Alzheimer's Disease, cancer, and atherosclerosis. Early reports showed that O₂^•- is a stimulant for collagen synthesis. However, the mechanism remains incompletely understood. Here we showed that LY83583 (6-anilinoquinoline-5,8-quinone), a substance known to induce O₂^•- production by smooth muscle cell (SMC), increases Type I collagen secretion. This effect could be blocked by treating the cells with Tiron, a scavenger for O₂^•-. LY83583-induced Type I collagen secretion required P4HA1 and P4HA2. Knockout of either P4ha1 or P4ha2 greatly reduced LY83583-stimulated Type I collagen maturatio

SUBMITTER: Shi R 

PROVIDER: S-EPMC7442856 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature