Validating Fluorescent Chrnb4.EGFP Mouse Models for the Study of Cone Photoreceptor Degeneration.

ABSTRACT:

Purpose

To validate the application of a known transgenic mouse line with green fluorescent cones (Chrnb4.EGFP) to study cone photoreceptor biology and function in health and disease.

Methods

Chrnb4.EGFP retinas containing GFP⁺ cones were compared with retinas without the GFP transgene via immunohistochemistry, quantitative real-time polymerase chain reaction, electroretinograms, and flow cytometry. The Chrnb4.EGFP line was backcrossed to the mouse models of cone degeneration, Pde6c^cpfl1 and Gnat2^cpfl3 , generating the new lines Gnat2.GFP and Pde6c.GFP, which were also studied as described.

Results

GFP expression spanned the length of the cone cell in the Chrnb4.EGFP line, as well as in the novel Gnat2

SUBMITTER: Brunet AA 

PROVIDER: S-EPMC7442867 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature