Composition of the murine gut microbiome impacts humoral immunity induced by rabies vaccines.

ABSTRACT:

Background

Gut microbiome plays a crucial role in modulating human and animal immune responses. Rabies is a fatal zoonosis causing encephalitis in mammals and vaccination is the most effective method to control and eliminate rabies. The relationship between the gut microbiome and humoral immunity post rabies vaccination has not been investigated yet.

Methods

Mice orally administrated with a cocktail of broad-spectrum antibiotics were inoculated with rabies vaccines, and humoral immune response was analyzed at indicated time points. The 16S ribosomal RNA (16S rRNA) gene sequencing was performed on fecal samples from groups in vancomycin-treated and untreated mice. Mice were immunized with rabies vaccines and virus-neutralizing antibody (VNA) levels were measured, resulting in

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7443138 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature