Evaluation of different commercial antibodies for their ability to detect human and mouse tissue factor by western blotting.

ABSTRACT:

Background

Western blotting is used to measure protein expression in cells and tissues. Appropriate interpretation of resulting data is contingent upon antibody validation.

Objectives

We assessed several commercial anti-human and anti-mouse tissue factor (TF) antibodies for their ability to detect TF by western blotting.

Material and methods

We used human pancreatic cancer cell lines expressing different levels of TF and a mouse pancreatic cancer cell line expressing TF with a matched knockout derivative.

Results

Human and mouse TF protein detected by western blotting correlated with levels of TF mRNA in these cell lines. The apparent molecular weight of TF is increased by N-linked glycosylation and, as expected, deglycosylation decreased the size of TF based o

SUBMITTER: Rosell A 

PROVIDER: S-EPMC7443430 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature