Improving diagnosis of von Willebrand disease: Reference ranges for von Willebrand factor multimer distribution.

ABSTRACT:

Background

Phenotypic von Willebrand disease (VWD) classification requires multiple tests including analysis of multimeric distributions von Willebrand factor (VWF) and evaluation of its structure. VWF multimer analysis is labor intensive, nonstandardized, and limited to specialized laboratories. A commercial semiautomatic assay, HYDRAGEL VW multimer assay (H5/11VWM, Sebia), has become available.

Objectives

Establishment of reference ranges for H5/11VWM to improve VWD classification.

Methods

Implementation validation, establishment and validation of normal and pathological reference intervals (NRIs/PRIs), comparison with in-house method using 40 healthy volunteers and 231 VWD patients.

Results

Qualitative and quantitative validation of NRI obtained sensitivity

SUBMITTER: Vangenechten I 

PROVIDER: S-EPMC7443431 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature