Establishment of Immortalized Laryngeal Epithelial Cells Transfected with Bmi1.

ABSTRACT: Primary laryngeal epithelial cells are essential to exploring the mechanisms of laryngeal and voice disorders; however, they are difficult to study and apply because of their limited life span. The purpose of this study was to develop a stable and reliable in vitro model for the comprehensive study of the pathogenesis of laryngeal and voice diseases. The pLVTHM-Bmi1 plasmid was constructed and used to immortalize primary laryngeal epithelial cells by lentiviral infection. The expressions of Bmi1, human telomerase reverse transcriptase (hTERT), p53, and pRB pathway proteins were detected by western blotting. Functional characteristics of the immortalized cell lines were verified by cell senescence β-galactosidase staining, 5-ethynyl-2'-deoxyuridine cell proliferation test, and flow c

SUBMITTER: Tan JJ 

PROVIDER: S-EPMC7444206 | biostudies-literature | 2020 Jan-Dec

REPOSITORIES: biostudies-literature