Engineered Stem Cells Improve Neurogenic Bladder by Overexpressing SDF-1 in a Pelvic Nerve Injury Rat Model.

ABSTRACT: There is still a lack of sufficient research on the mechanism behind neurogenic bladder (NB) treatment. The aim of this study was to explore the effect of overexpressed stromal cell-derived factor-1 (SDF-1) secreted by engineered immortalized mesenchymal stem cells (imMSCs) on the NB. In this study, primary bone marrow mesenchymal stem cells (BM-MSCs) were transfected into immortalized upregulated SDF-1-engineered BM-MSCs (imMSCs/eSDF-1⁺) or immortalized normal SDF-1-engineered BM-MSCs (imMSCs/eSDF-1^-). NB rats induced by bilateral pelvic nerve (PN) transection were treated with imMSCs/eSDF-1⁺, imMSCs/eSDF-1^-, or sham. After a 4-week treatment, the bladder function was assessed by cystometry and voiding pattern analysis. The

SUBMITTER: Zhu GQ 

PROVIDER: S-EPMC7444235 | biostudies-literature | 2020 Jan-Dec

REPOSITORIES: biostudies-literature