A novel function of FAF1, which induces dopaminergic neuronal death through cell-to-cell transmission.

ABSTRACT:

Background

Fas-associated factor 1 (FAF1) has been implicated in Parkinson's disease (PD) and activates the cell death machinery in the cytosol. However, the presence of extracellular FAF1 has not been studied.

Methods

Serum-free conditioned medium (CM) from FAF1-transfected SH-SY5Y cells was concentrated and analyzed by western blotting. Exosomes were isolated from CM by ultracentrifugation and analyzed by western blotting, electron microscopy and nanoparticle tracking analysis. Soluble FAF1 from CM was immunodepleted using anti-FAF1 antibody. Transmission of secreted FAF1 was examined by transwell assay under a confocal microscope. CM-induced cell death was determined by measuring propidium iodide (PI) uptake using a flow cytometer.

Results

FAF1 was secreted from SH

SUBMITTER: Park G 

PROVIDER: S-EPMC7444258 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature