Characterization and internalization of small extracellular vesicles released by human primary macrophages derived from circulating monocytes.

ABSTRACT: Extracellular vesicles (EVs) are small membrane-limited structures derived from outward budding of the plasma membrane or endosomal system that participate in cellular communication processes through the transport of bioactive molecules to recipient cells. To date, there are no published methodological works showing step-by-step the isolation, characterization and internalization of small EVs secreted by human primary macrophages derived from circulating monocytes (MDM-derived sEVs). Thus, here we aimed to provide an alternative protocol based on differential ultracentrifugation (dUC) to describe small EVs (sEVs) from these cells. Monocyte-derived macrophages were cultured in EV-free medium during 24, 48 or 72 h and, then, EVs were isolated from culture supernatants by (dUC). Macrophages s

SUBMITTER: Arteaga-Blanco LA 

PROVIDER: S-EPMC7444811 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature