Global identification of a marine diatom long noncoding natural antisense transcripts (NATs) and their response to phosphate fluctuations.

ABSTRACT: Often ignored and regarded as mere transcriptional noise, long noncoding RNAs (lncRNAs) are starting to be considered key regulators of gene expression across the Eukarya domain of life. In the model diatom Phaeodactylum tricornutum, we have previously reported the occurrence of 1,510 intergenic lncRNAs (lincRNAs), many of which displaying specific patterns of expression under phosphate fluctuation (Pi). Using strand-specific RNA-sequencing data we now expand the repertoire of P. tricornutum lncRNAs by identifying 2,628 novel natural antisense transcripts (NATs) that cover 21.5% of the annotated genomic loci. We found that NAT expression is tightly regulated by phosphate depletion and other naturally occurring environmental stresses. Furthermore, we identified 121 phosphate stress responsi

SUBMITTER: Cruz de Carvalho MH 

PROVIDER: S-EPMC7445176 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature