Clonal array profiling of scFv-displaying phages for high-throughput discovery of affinity-matured antibody mutants.

ABSTRACT: "Antibody-breeding" approach potentially generates therapeutic/diagnostic antibody mutants with greater performance than native antibodies. Therein, antibody fragments (e.g., single-chain Fv fragments; scFvs) with a variety of mutations are displayed on bacteriophage to generate diverse phage-antibody libraries. Rare clones with improved functions are then selected via panning against immobilized or tagged target antigens. However, this selection process often ended unsuccessful, mainly due to the biased propagation of phage-antibody clones and the competition with a large excess of undesirable clones with weaker affinities. To break radically from such panning-inherent problems, we developed a novel method, clonal array profiling of scFv-displaying phages (CAP), in which colonies of the i

SUBMITTER: Kiguchi Y 

PROVIDER: S-EPMC7445280 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature