Bluetongue virus viral protein 7 stability in the presence of glycerol and sodium chloride.

ABSTRACT:

Purpose

The Orbivirus Bluetongue virus (BTV) is an economically significant disease that affects mainly wild and domestic ruminants. BTV is most often seen symptomatically in sheep, but is easily carried by goats, cattle, and wild ruminants. To date there are several problems with the vaccines currently available for BTV, and one of the most promising candidates to increase vaccine efficacy is a protein-based vaccine, for which viral protein 7 (VP7) is a great candidate to be included in it. In order to further these studies, the stability of BTV VP7 in common vaccine additives needs to be investigated.

Materials and methods

Recombinant BTV VP7 was expressed in a bacterial cell system and purified before being analysed using spectroscopic techniques including far-ultr

SUBMITTER: Russell BL 

PROVIDER: S-EPMC7445327 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature