Transcriptional and Functional Analysis of CD1c⁺ Human Dendritic Cells Identifies a CD163⁺ Subset Priming CD8⁺CD103⁺ T Cells.

ABSTRACT: Dendritic cells (DCs) are antigen-presenting cells controlling T cell activation. In humans, the diversity, ontogeny, and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88^-CD1c⁺CD163⁺ DCs (called DC3s) as immediate precursors of inflammatory CD88^-CD14⁺CD1c⁺CD163⁺FcεRI⁺ DCs. DC3s develop via a specific pathway activated by GM-CSF, independent of cDC-restricted (CDP) and monocyte-restricted (cMoP) progenitors. Like classical DCs but unlike monocytes, DC3s drove activation of naive T cells. In vitro, DC3s displayed a distinctive ability to prime CD8⁺ T cells expressing a tissue homing signature and the epithelial homing alpha-E integrin (CD103)

SUBMITTER: Bourdely P 

PROVIDER: S-EPMC7445430 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature