Imaging Biotin Trafficking In Vivo with Positron Emission Tomography.

ABSTRACT: The water-soluble vitamin biotin is essential for cellular growth, development, and well-being, but its absorption, distribution, metabolism, and excretion are poorly understood. This paper describes the radiolabeling of biotin with the positron emission tomography (PET) radionuclide carbon-11 ([¹¹C]biotin) to enable the quantitative study of biotin trafficking in vivo. We show that intravenously administered [¹¹C]biotin is quickly distributed to the liver, kidneys, retina, heart, and brain in rodents-consistent with the known expression of the biotin transporter-and there is a surprising accumulation in the brown adipose tissue (BAT). Orally administered [¹¹C]biotin was rapidly absorbed in the small intestine and swiftly distributed to the same organs. Pre

SUBMITTER: Bongarzone S 

PROVIDER: S-EPMC7445742 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature