Engineering a living biomaterial via bacterial surface capture of environmental molecules.

ABSTRACT: Synthetic biology holds significant potential in biomaterials science as synthetically engineered cells can produce new biomaterials, or alternately, can function as living components of new biomaterials. Here, we describe the creation of a new biomaterial that incorporates living bacterial constituents that interact with their environment using engineered surface display. We first developed a gene construct that enabled simultaneous expression of cytosolic mCherry and a surface-displayed, catalytically active enzyme capable of covalently bonding with benzylguanine (BG) groups. We then created a functional living material within a microfluidic channel using these genetically engineered cells. The material forms when engineered cells covalently bond to ambient BG-modified molecules upon ind

SUBMITTER: Scott FY 

PROVIDER: S-EPMC7445765 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature