Rapid production and characterization of antimicrobial colicins using Escherichia coli-based cell-free protein synthesis.

ABSTRACT: Colicins are antimicrobial proteins produced by Escherichia coli, which, upon secretion from the host, kill non-host E. coli strains by forming pores in the inner membrane and degrading internal cellular components such as DNA and RNA. Due to their unique cell-killing activities, colicins are considered viable alternatives to conventional antibiotics. Recombinant production of colicins requires co-production of immunity proteins to protect host cells; otherwise, the colicins are lethal to the host. In this study, we used cell-free protein synthesis (CFPS) to produce active colicins without the need for protein purification and co-production of immunity proteins. Cell-free synthesized colicins were active in killing model E. coli cells with different modes of cytotoxici

SUBMITTER: Jin X 

PROVIDER: S-EPMC7445778 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature