Translation inhibition and resource balance in the TX-TL cell-free gene expression system.

ABSTRACT: Quantifying the effect of vital resources on transcription (TX) and translation (TL) helps to understand the degree to which the concentration of each resource must be regulated for achieving homeostasis. Utilizing the synthetic TX-TL system, we study the impact of nucleotide triphosphates (NTPs) and magnesium (Mg²⁺) on gene expression. Recent observations of the counter-intuitive phenomenon of suppression of gene expression at high NTP concentrations have led to the speculation that such suppression is due to the consumption of resources by TX, hence leaving fewer resources for TL. In this work, we investigate an alternative hypothesis: direct suppression of the TL rate via stoichiometric mismatch in necessary reagents. We observe NTP-dependent suppression even in the early pha

SUBMITTER: Nagaraj VH 

PROVIDER: S-EPMC7445783 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature